Abstract
Twentyfour-hour variation in (14 C) acetate incorporation in the liver lipid, and lipid concentrations in the liver and muscle tissues were studied during different phases of the annual reproductive cycle in the female freshwater catfish, H. fossilis. A 24-h rhythm in hepatic lipogenic activity was validated. Lipid concentration in liver and muscle also varied in a rhythmic fashion (t = 24 h). The mesor and amplitude of these rhythms appeared to be modulated by the phase of the annual gonadal cycle of H. fossilis. All three lipid parameters, in addition, exhibited low frequency rhythms with a t = 6 months or 3 months. The results indicate that the time of the day and month of the year for sampling in any experiment involving examination of lipid metabolism are of critical importance.
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