Diuretic and Antidiuretic Activities of Ethanol Extract and Fractions of Lagopsis supina in Normal Rats.

Zhiyong Liu,Rongrui Wei,Xiaoquan Luo,Longxue Li,Li Yang,Zejing Mu,Yun Huang,Tingting Xu,Junwei He

doi:10.1155/2019/6927374

Zhiyong Liu, Rongrui Wei + Show 7 more

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https://doi.org/10.1155/2019/6927374

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Abstract

Lagopsis supina is a well-known traditional Chinese medicine and used as an agent for diuresis in China for centuries. This is the first time to evaluate the diuretic activity of the ethanol extract of L. supina (LS) and its four fractions (LSA, LSB, LSC, and LSD) in normal rats. After the administration of LS-H, LS-M, LSB-H, and LSC-L, the urine output of the rats was significantly increased, while the urine excretion was significantly reduced after treatment with LSB-L. The urine Na+ excretion was remarkably increased with LS-H, LS-M, LSA-H, LSA-L, LSB-H, LSC-L, and LSD-L, and the urine K+ excretion was significantly increased after administration of LS-H and LSB-H. Moreover, the urine Na+ and K+ excretion was significantly reduced after treatment with LSC-H and LSD-H. However, the urine pH values and urine and serum Na+-K+-ATPase levels did not show remarkable change after administration of LS or its four fractions in comparison with the control group. On the contrary, LS and its four fractions can suppress the renin-angiotensin-aldosterone system (RAAS), including ADH arrest by LSB-H, LSB-L, LSC-L, LSD-L, and LSD-H and ALD arrest by LSD-L, as well as promote ANP release by LS-M, LSB-H, LSC-H, and LSD-H, while furosemide can suppress only arrest of ADH within 24 h compared with the control group. In addition, LS and its four fractions did not change the urine and serum TNF-α and IL-6 levels in normal rats within 24 h. This study will provide a quantitative basis for explaining the natural medicinal use of LS as a diuretic agent for edema and promoting the diuretic process.

Highlights

Natural medicine is considered a quite important resource for drug discovery and has increasingly attracted the attention of researchers [1,2,3,4]
To determine whether the ethanol extract and its four fractions (LSA, LSB, LSC, and LSD) of L. supina (LS) have the diuretic effects, we measured the urinary excretion volume in every group at different time points (1, 2, 4, 6, and 24 h) after treatment with LS and its four fractions or furosemide. e urine output volume increased significantly (P < 0.05 or P < 0.01) for LSA-L (19.3 mg/kg), LS-L (114.5 mg/kg), and LS-M (229.0 mg/kg) within 1, 2, and 4 h, respectively, while LSD-H (13.6 mg/kg) produced a remarkable reduction in the urinary excretion volume within 4 h after administration compared with the control group (P < 0.05, Table 1)
Urinary excretion volume in LS-H (458.0 mg/kg), LS-M (229.0 mg/kg), LSB-H (322.6 mg/kg), and LSC-L (8.8 mg/kg) groups significantly increased within 24 h after administration (P < 0.05 or P < 0.01), while LSB-L (80.9 mg/kg) produced a remarkable reduction in the urinary excretion volume within 24 h after administration (P < 0.01, Table 1)

Summary

Introduction

Natural medicine is considered a quite important resource for drug discovery and has increasingly attracted the attention of researchers [1,2,3,4]. In Chinese folk medicine, several traditional Chinese medicines (TCMs) are used as diuretic drugs, but most of them lack pharmacological studies to explain their material basis and its mechanisms [5]. The pharmacological research of L. supina was more, the diuretic activity of LS in normal rats has not yet been studied so far. We found that the ethanol extract of L. supina showed a significant diuretic activity in a rat model of traumatic blood stasis (TBS) [13].

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