Diuretic activity of phytosubstances from vaccinium vitis-idaea leaves

Abstract

The purpose of the work was to study the diuretic activity of phytosubstances from Vaccinium vitis-idaea leaves. Materials and methods . The objects of the study were 13 phytosubstanses, obtained from the leaves of Vaccinium vitis-idaea . The study of the effect on the excretory renal function was performed on intact white rats using the method E. B. Berchin. The study and analysis of experimental data was performed in comparison with the reference product of 25 mg/kg hypothiazide (Hipotiazide 25 mg No. 20, manufactured by Sanofi-aventis, Hungary). Results .The most pronounced diuretic effect showed phytosubstance from the leaves of Vaccinium vitis-idaea , which contains a complex of phenolic compounds with arginine, which at a dose of 100 mg/kg increased diuresis three times and has dose-dependent effect. It has been established that amino acids in combination with phenolic compounds of cranberries usually potentiate the diuretic effect of the extract. The polysaccharide complex of common cranberry leaves manifested itself as an antidiuretic agent, and the presence of polysaccharides in the extracts reduced the diuretic effect. The aglycones of the phenolic compounds of the Vaccinium vitis-idaea leaves had a less pronounced diuretic effect than their glycosides. Conclusions . The study of diuretic activity of 13 phytosubstanses from the leaves of Vaccinium vitis-idaea was conducted. The most promising substance was a complex of glycosides of phenolic compounds of Vaccinium vitis-idaea with arginine. It has been established that amino acids in combination with phenolic compounds of Vaccinium vitis-idaea affect the diuretic effect of the extract, potentiating it, and polysaccharides, on the contrary, inhibit it. Glycosides of phenolic compounds of Vaccinium vitis-idaea leaves had a more pronounced diuretic effect than their aglycones.