Distyly and variation in heteromorphic Incompatibility in Gaertnera Vaginata (Rubiaceae) endemic to La Reunion Island

Abstract

Documenting the floral biology of species throughout the Rubiaceae family is of particular interest since heterostyly and dioecy may have evolved more than once in this large family. Unfortunately many species in several tropical regions remain unstudied. The purpose of this paper is to describe the floral biology, the nature of self-incompatibility, morph ratios, and fecundity in natural populations of Gaertnera vaginata, a small tree endemic to the island of La Reunion in the Indian Ocean. Measurements of floral characters in populations across the entire distribution of this species showed that G. vaginata exhibits a reciprocal stigma height and anther height dimorphism characteristic of a distylous species. Pollen grain size and corolla tube length are consistently greater in short-styled plants and long-styled plants produce more pollen per flower. Controlled pollinations in a natural population showed that 25% of the short-styled plants gave at least one fruit on intramorph (illegitimate) pollination, whereas no long-styled plants set fruit on illegitimate pollination. In total, 19.4% of illegitimate pollinations produced fruit on short-styled plants. No self-pollination gave fruit on either morph and between-morph pollinations produced 92.2 and 92.8% for short and long-styled plants, respectively. Overall, short-styled plants were significantly more abundant than long-styled plants. Short-styled plants outnumbered long-styled plants in 16 of the 19 populations. In three of these populations the morph ratio was significantly different from 1:1. In two natural populations, fruit set was significantly higher on long-styled plants, although the number of seeds per fruit was not significantly different between the two morphs. The possible effect of variation in the strength of heteromorphic incompatibility on observed variation in morph abundance and the possible causes for the variation in fruit set are discussed.