Abstract
Abstract A survey indicated that rhizobia capable of nodulating white clover (Trifolium repens L.) are absent from large areas of undeveloped tussock grassland in Otago. The only areas where the presence of rhizobia can be predicted are those where T. arvense L. (haresfoot trefoil) or other naturalised clovers are present. Rhizobia isolated from nodules of clover sown on soils containing naturalised populations of rhizobia were tested for their effectiveness of nitrogen fixation with ‘Grasslands Huia’ white clover. Effectiveness of the isolates ranged from 2–138% of the effectiveness of strains recommended for inoculation. Mean effectiveness of the strains from individual sites ranged from 46% to 93%. The use of three anti-Rhizobium fluorescent antibodies of three inoculant strains (PDDCC 2153, 4144, and 2163) identified a minimum of 10 serological types among R. trifolii isolated from a tussock grassland soil and a soil under a permanent ryegrass/white clover pasture. A maximum of 22% of the isolates wer...
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