Abstract
The plasmalemmal structure of satellite cells in the lumbar spinal ganglia of rat was examined by freeze-fracture and by a cytochemical method for the demonstration of alkaline phosphatase activity. Plasma membranes of satellite cells are the only ones in the ganglia to contain, in addition to globular intramembrane particles, orthogonal arrays of particles 6-7 nm in diameter. The arrays are most concentrated in the portions of the membranes contacting the basal lamina, or outer membranes; they decrease considerably in number in lateral membranes, and are rare in the membrane regions adjacent to the neuronal perikaryon, or inner membranes. Such gradual decrease in array density in satellite cells suggests regional differences of plasma membrane properties within the same cell. Alkaline phosphatase, which was chosen as a cytochemical marker for membrane activity because of its relation to transport function, localizes to inner and lateral membranes, and not to outer membranes of satellite cells. The absence of correlation between localization of orthogonal arrays and such enzymatic activity suggests that the membranes provided with many arrays possess some characteristics different from other membranes that may exclude transport activity. The possible significance of orthogonal arrays and their close association with the basal lamina are discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
