Distribution of wingless type 3a (Wnt3a) Rs 752107 gene polymorphism on orofacial cleft patients

Abstract

Non-syndromic orofacial cleft, including cleft lip with or without cleft on palate (CB +/- CL NS) is the most common disorder resulting from craniofacial development disorder during pregnancy. These developmental abnormalities occur due to interference during the fusion stage or time and position disturbance of the processes and/or palatal shelves. The prevalence of orofacial cleft is around 1:500 - 1:2000 in the world. Wnt3a, which is the genes belonging to the Wnt gene family controls craniofacial development during pregnancy. It involves regulating the development of the middle face and upper lip fusion. Therefore this gene plays an important role as a cause in non-syndromic cleft lip/palate. The relationship of Wnt3a gene polymorphism to the orofacial cleft occurrence in Indonesia remains unclear. To examine the relationship of polymorphism of Wnt3a Rs 752107 genes in orofacial cleft patients in Indonesia. The study samples are raw material stored in the Oral Biology Laboratory of the Faculty of Dentistry, the University of Indonesia in the form of DNA from cleft lip patients. Study on the distribution of genetic variation of wnt3a rs 752107 in 30 samples of CB +/- CL NS patients and 170 control samples without orofacial cleft using Polymerase Chain Reaction (PCR-RFLP) examination with Alu1 enzyme. Significance of variation difference test using Chi-square test on SPSS 22. Frequency of CC were dominant in both samples (>90%). There was no significant difference between gene polymorphisms of wnt3a rs 752107 in orofacial cleft with control (p>0.05)