Distribution of transcribable DNA sequences in mouse liver hepatoma chromatin.

Abstract

Shearing in either a Virtis small or large volume capacity cup under defined conditions gave chromatin fragment size distribution upon agarose exclusion column chromatography (50 × 106) that varied inversely with shear time. The large- and small-sized chromatin fragments possessed high template capacity while the intermediate fragments (the majority) had relatively low template capacity; the differences in template capacity of these size groups varying inversely with the shear time. The protein/DNA and RNA/DNA ratios remained relatively constant in all size groups.