Abstract
The cfiA gene, encoding an imipenem-hydrolyzing metallo-beta-lactamase produced by Bacteroides fragilis, and insertion-like elements were detected by PCR amplification with B. fragilis strains isolated in Japan. The cfiA gene was found in 1.9 and 4.1% of the imipenem-susceptible B. fragilis isolates collected from 1987 to 1988 and from 1992 to 1994, respectively. Insertion-like elements adjacent to the cfiA gene were found in all nine metallo-beta-lactamase-producing imipenem-resistant strains tested but not in nine cfiA-positive strains with no detectable metallo-beta-lactamase activity.
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