Distribution of sympathetic preganglionic neurons and monoaminergic nerve terminals in the spinal cord of the rat

Abstract

A study was made of the distribution of sympathetic preganglionic neurons identified by retrograde labeling with horseradish peroxidase from various peripheral nerve trunks and of the distributions of monoaminergic terminals in the spinal cord of the rat. Nerve terminals were stained immunohistochemically by using antisera raised against tyrosine hydroxylase, phenylethanolamine-N-methyl-transferase, neuropeptide Y, and 5-hydroxytryptamine and by using formaldehyde-induced fluorescence. The three-dimensional distribution of sympathetic preganglionic neurons was described by using computer reconstruction and compared with the arrangement of each population of immunohistochemically stained terminals in the intermediate zone. Although monoaminergic terminals are associated with most sympathetic neurons, particularly in the intermediolateral column, the relationship of many terminals to sympathetic neuron somata in other parts of the intermediate zone is tenuous. Some of the descending innervation may terminate on interneurons. The data are consistent with the coexistence of phenylethanolamine-N-methyl-transferase and neuropeptide Y in terminals arising from cell bodies in the C1 region in the ventrolateral medulla and with the presence of at least two populations of catecholaminergic terminals as well as the adrenergic one. Serotoninergic terminals are denser and have a different arrangement from those of catecholaminergic terminals in the intermediate zone.