Abstract
The presence of PCB-TRB and PCB-TOB in the Baltic, Bering, and Chukchi seas was determined. It was found that most of these physiological groups of bacteria reach a chronically contaminated PCB marine ecosystem of the Baltic Sea. It was revealed that the number of PCB-TRB and TCB-TOB mainly depends on the concentration of PCBs in the suspended matter. The dependence manifests itself against the background of the impact of other abiotic environmental factors—temperature and salinity, as well as the content of various forms of phosphorus and nitrogen.
Highlights
Ìàòåðèàëû è ìåòîäûÌàòåðèàëîì äëÿ íàñòîÿùåé ðàáîòû ïîñëóæèëè äàííûå êîìïëåêñíûõ ýêîëîãè÷åñêèõ ýêñïåäèöèé, ïîëó÷åííûå â 2001—2004 ãã. â Áàëòèéñêîì è ×óêîòñêîì ìîðÿõ, à òàêæå â Àíàäûðñêîì çàëèâå Áåðèíãîâà ìîðÿ
Èçó÷åíèå ìîðñêèõ ÏÕÁ-ÒÐÁ è ÏÕÁ-ÒÎÁ íà÷àëîñü ñðàâíèòåëüíî íåäàâíî [4, 5], à èíôîðìàöèÿ î âëèÿíèè ôàêòîðîâ îêðóæàþùåé ñðåäû íà ÷èñëåííîñòü áàêòåðèé ýòèõ ãðóïï â ìîðñêèõ ýêîñèñòåìàõ ïðàêòè÷åñêè ïîëíîñòüþ îòñóòñòâóåò
Biodegradative potential and characterization of psychrotolerant polychlorinated biphenyl degrading marine bacteria isolated from a coastal station in the Terra Nova Bay
Summary
Ìàòåðèàëîì äëÿ íàñòîÿùåé ðàáîòû ïîñëóæèëè äàííûå êîìïëåêñíûõ ýêîëîãè÷åñêèõ ýêñïåäèöèé, ïîëó÷åííûå â 2001—2004 ãã. â Áàëòèéñêîì è ×óêîòñêîì ìîðÿõ, à òàêæå â Àíàäûðñêîì çàëèâå Áåðèíãîâà ìîðÿ. Äëÿ ó÷åòà ÷èñëåííîñòè ÏÕÁ-ÒÐÁ áàêòåðèé ïðèìåíÿëè ñðåäó ÌÌÑ [6; 7] ñëåäóþùåãî ñîñòàâà (ã): NaCl — 5,0; MgSO4 1⁄2 7H20 — 1,0; KCl — 0,7; K2HPO4 — 2,0; Na2HPO4 — 3,0; NH4NO3 — 1,0; âîäà äèñòèëëèðîâàííàÿ — 1,0 ë. Äëÿ ïîëó÷åíèÿ ìàòî÷íîãî ðàñòâîðà ÏÕÁ íàâåñêó â 1 ìã ñóáñòðàòà ðàñòâîðÿëè â 1 ìë àöåòîíà, ðàñòâîð âíîñèëè â 100 ìë ñòåðèëüíîé ñðåäû ÌÌÑ. Äëÿ îïðåäåëåíèÿ ÷èñëåííîñòè ÏÕÁ-ÒÎÁ â ïðîáèðêè ñî ñðåäîé ÌÌÑ íàðÿäó ñ ÏÕÁ âíîñèëè 1—2 êàïëè ñòåðèëüíîãî 10%-ãî ðàñòâîðà ãëþêîçû [8]. Äëÿ ó÷åòà ÷èñëåííîñòè ñàïðîòðîôíûõ áàêòåðèé ïðèìåíÿëè ðàçáàâëåííûé ñòåðèëüíûé ðûáíûé áóëüîí, ïðèãîòîâëåííûé íà ìîðñêîé âîäå èç ìåñòà âçÿòèÿ ïðîáû [8]. Ïðè ïîñëåäóþùåì àíàëèçå ôèëüòðîâ ñî âçâåñüþ ìåòîäîì âûñîêîýôôåêòèâíîé ãàçîæèäêîñòíîé õðîìàòîãðàôèè (ÂÝÆÕ) îïðåäåëÿëè ñîäåðæàíèå ÏÕÁ âî âçâåøåííîì âåùåñòâå, à ñ ó÷åòîì îáúåìà ïðîôèëüòðîâàííîé âîäû ðàññ÷èòûâàëè êîíöåíòðàöèþ âçâåøåííîãî ÏÕÁ â âîäå. Ñòàòèñòè÷åñêèå àíàëèçû ïðîâîäèëè íà ïåðñîíàëüíîì êîìïüþòåðå ñ èñïîëüçîâàíèåì ïàêåòîâ ïðîãðàìì Statistica 6.0 è Excel 2002
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