Abstract
Platelet activating factor (PAF) receptor mRNA was semi-quantitated in microdissected rat nephron segments by the reverse transcription-polymerase chain reaction (RT-PCR). PCR primers were designed from two alternative splicing isoforms of rat PAF receptor cDNA; a homologue of human leukocyte PAF receptor cDNA (p172) and the other with a unique sequence in the 5′-noncoding region upstream of the splice acceptor site (p18). Only the expression of mRNA corresponding to p172 was detectable, which was most abundant in the glomerulus, followed in order by: proximal convoluted tubule > proximal straight tubule > outer medullary and cortical collecting duct, distal convoluted tubule > thick ascending limb of Henle's loop. These results suggest the potential physiological roles of PAF in the entire renal tubule as well as in the glomerulus.
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