Abstract
The variants distribution of polymorphic sites -1026G>A and -369G>C of the p21 gene in gastric cancer according to tumor histological type (intestinal or diffuse gastric cancer) and the tumor stage (with or without metastases) was investigated. DNA samples isolated from the morphologically intact stomach tissue obtained from the resection margin from 200 gastric cancer patients via standard methods, were studied for polymorphisms -1026G>A and -369G>C of the p21 gene by real-time polymerase chain reaction with hybridization-fluorescence detection using the appropriate pairs of oligonucleotide primers and probes. The carriage of allele A and genotype AA of the polymorphism -1026G>A, as well as allele C and genotype CC of the polymorphism -369G>C of the p21 gene, were predominantly associated with diffuse histological type of gastric cancer. In the cohort of patients with metastatic gastric cancer, the frequencies of allele C, genotypes GC and CC of the polymorphism -369G>C, and allele A, genotype AA of the polymorphism -1026G>A of the p21 gene, were statistically higher compared to the corresponding parameters of patients with non-metastatic gastric cancer.
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