Abstract
The repeat sequences occupied more than 50 % of soybean genome. In order to understand where these repeat sequences distributed in soybean genome and its related Glycine species, we examined three new repeat sequences—soybean repeat sequence (SBRS1, SBRS2 and SBRS3), some nonspecific repeat sequences and 45S rDNA on several Glycine species, including annual and perennial accessions in this study. In the annual species, G. soja, signals for SBRS1 and ATT repeat can be found on each chromosome in GG genome, but those for SBRS2 and SBRS3 were located at three specific loci. In perennial Glycine species, these three SBR repeat frequently co-localized with 45S rDNA, two major 45S rDNA loci were found in all tetraploid species. However, an extra minor locus was found in one accession of the G. pescadrensis (Tab074), but not in another accession (Tab004). We demonstrate that some repetitive sequences are present in all Glycine species used in the study, but the abundancy is different in annual or perennial species. We suggest this study may provide additional information in investigations of the phylogeny in the Glycine species.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0103-9) contains supplementary material, which is available to authorized users.
Highlights
IntroductionWild germplasm may be a significant breeding resource for current varieties that have undergone several genetic bottlenecks and have shown limited genetic variability (Hyten et al 2006; Keim et al 1990)
We chose 3 new repeat sequences, designated soybean repeat sequence1 (SBRS1), SBRS2 and SBRS3; their sequences and designed primers are in Additional files 1 and 2
The predicted repeat sequences were confirmed by cytological study The soybean WGS sequence project has set significantly new milestones for understanding the soybean genome and its evolutionary history (Jackson et al 2006; Schmutz et al 2010)
Summary
Wild germplasm may be a significant breeding resource for current varieties that have undergone several genetic bottlenecks and have shown limited genetic variability (Hyten et al 2006; Keim et al 1990). These wild germplasm harbor many traits of interest to soybean breeders, such as biotic and abiotic disease resistance (Newell and Hymowitz 1975), high seed protein content (Sebolt et al.2000) and increased yield (Concibido et al 2003). Investigating Glycine species is important for soybean genetics and breeding
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