Distribution of neurotensin-immunoreactivity within baroreceptive portions of the nucleus of the tractus solitarius and the dorsal vagal nucleus of the rat.

Abstract

We have examined the distribution of neurotensin immunoreactivity within subnuclear regions of the nucleus of the tractus solitarius (NTS) and the dorsal motor nucleus of the vagus nerve (DVN) in the rat. In order to determine which regions of the NTS were involved in the regulation of baroreceptor reflexes, we mapped the central distribution of the aortic branch of the vagus nerve using transganglionic transport of horseradish peroxidase. Comparison of the pattern of aortic nerve innervation with that of the distribution of neurotensin-immunoreactive cells and fibers shows the dorsomedial nucleus of the NTS both to be the primary site of aortic baroreceptor termination and to contain the highest concentration of neurotensin-immunoreactive elements within the NTS. Neurotensin-immunoreactive fibers are also present in medial regions of the NTS adjacent to the area postrema where they may be involved in the modulation of vagal gastric afferents. Double-label experiments, in which, on the same tissue sections, neurotensin immunohistochemistry was combined with retrograde horseradish peroxidase labeling of DVN neurons, reveal a topographic innervation of vagal preganglionic motoneurons by neurotensin-immunoreactive fibers. The heaviest innervation is of lateral portions of the DVN and adjacent ventral portions of the NTS at the level of the obex, an area which may contain cardiac motoneurons. In this region neurotensin-immunoreactive fibers can be observed in close proximity to retrogradely labeled cells. The concentration of neurotensin elements in a region of the NTS which is involved in the control of baroreceptor reflexes provides a morphological basis for the cardiovascular effects produced by central administration of the peptide. Additional control may be exerted at the level of the motoneuron, as evidenced by apparent neurotensin fiber innervation of presumptive cardiac preganglionic neurons. Similarly, the distribution of neurotensin fibers suggests that the peptide may be acting in gastric regulatory areas of the NTS or on vagal secretomotor neurons to regulate gastric acid secretion.