Abstract
Reconstructing the lineage of cells is central to understanding how the wide diversity of cell types develops. Here, we provide the neurosensory lineage reconstruction of a complex sensory organ, the inner ear, by imaging zebrafish embryos in vivo over an extended timespan, combining cell tracing and cell fate marker expression over time. We deliver the first dynamic map of early neuronal and sensory progenitor pools in the whole otic vesicle. It highlights the remodeling of the neuronal progenitor domain upon neuroblast delamination, and reveals that the order and place of neuroblasts' delamination from the otic epithelium prefigure their position within the SAG. Sensory and non-sensory domains harbor different proliferative activity contributing distinctly to the overall growth of the structure. Therefore, the otic vesicle case exemplifies a generic morphogenetic process where spatial and temporal cues regulate cell fate and functional organization of the rudiment of the definitive organ.
Highlights
A major challenge in developmental biology is to explain how spatiotemporally controlled cell specification and differentiation occur alongside morphogenesis in the construction of functional organs
The key cell types of the inner ear, the supporting cells and the hair cells of the sensory patches, and the sensory neurons that innervate them, originate early during embryonic development from progenitors located in the otic vesicle, a 3D-structure arising from the otic placode adjacent to the developing hindbrain (Durruthy-Durruthy et al, 2014; Raft et al, 2007; Sapede et al, 2012; Satoh and Fekete, 2005), and they are easy to score by morphology, position and specific markers (Haddon and Lewis, 1996; Raft and Groves, 2015; Whitfield, 2015; Wu and Kelley, 2012)
We have identified many of the genes that are important for setting these instructions, it is not known how the progenitor cells behave in the inner ear or how they follow these guidelines
Summary
A major challenge in developmental biology is to explain how spatiotemporally controlled cell specification and differentiation occur alongside morphogenesis in the construction of functional organs. The key cell types of the inner ear, the supporting cells and the hair cells of the sensory patches, and the sensory neurons that innervate them, originate early during embryonic development from progenitors located in the otic vesicle, a 3D-structure arising from the otic placode adjacent to the developing hindbrain (Durruthy-Durruthy et al, 2014; Raft et al, 2007; Sapede et al, 2012; Satoh and Fekete, 2005), and they are easy to score by morphology, position and specific markers (Haddon and Lewis, 1996; Raft and Groves, 2015; Whitfield, 2015; Wu and Kelley, 2012).
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