Abstract
The distribution of membrane-bound calcium and activated calmodulin was visualized during carrot somatic embryogenesis by chlorotetracycline (CTC) and fluphenazine fluorescence respectively. Somatic embryos of all stages possessed a higher CTC fluorescence in comparison with the signal from their precursors, the proembryogenic masses. The CTC fluorescence was evenly distributed in the somatic embryos. In contrast, fluphenazine was observed in some regions of the proembryogenic masses only. In the globular, heart-shaped and early torpedo-shaped stage its fluorescence was restricted to the basal part of the embryo. In the older torpedo-shaped embryos also the shoot apex showed fluphenazine fluorescence. It is concluded that during carrot somatic embryogenesis a polarity in the distribution of the activated calmodulin already exists before this polarity is morphologically expressed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
