Abstract
Sodhi, M., Mishra, B.P., Prakash, B., Kaushik, R., Singh, K.P. and Mukesh, M. 2010. Distribution of major allelic variants at exon-IV of kappa casein gene in Indian native cattle. J. Appl. Anim. Res., 38: 117–121. The present study was aimed to screen the pattern of major allelic variants of kappa casein (κ-CN) locus across 744 animals representing 17 Indian native cattle breeds adapted to different agroclimatic regions. A 935 bp fragment of κ-CN exon IV region was PCR amplified and digested with HindIII and HaeIII restriction enzymes to reveal the frequency distribution of A, B and E alleles. The digestion with HindIII pattern revealed a single 935 bp fragment or three fragments of 935, 520 and 415 bps, whereas HaeIII digestion displayed a monomorphic pattern of 641 and 294 bps fragments in all the studied samples. On analysis, only two alleles A and B were detected. AA genotype was most commonly distributed (0.818), with predominance of A allele (0.908). The present analysis indicated contrasting allelic pattern at κ-CN locus in Indian native cattle in comparison to that reported for Bos Taurus.
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