Distribution of local axon collaterals of mitral, displaced mitral, and tufted cells in the rabbit olfactory bulb.

Abstract

To determine the projection fields of intrabulbar axon collaterals, mitral, displaced mitral, and middle tufted cells in the rabbit olfactory bulb were stained by intracellular injection of HRP. The axon collaterals of mitral cells and displaced mitral cells were distributed exclusively within the granule cell layer (GrL). Those of middle tufted cells were distributed mostly in the GrL and on rare occasions in the mitral cell layer. None of these collaterals entered the external plexiform layer. Axon collaterals of mitral cells, emitted at the depths of the GrL, were distributed widely from the deep portion to the most superficial portion of the GrL. Collaterals of displaced mitral cells were also emitted in the deep part, but they tended to be distributed more superficially in the GrL than mitral cells. Collaterals of middle tufted cells were released and distributed superficially in the GrL. The axon collaterals of these principal cells typically extended for a longer distance than the secondary dendrites, and they sometimes formed bushlike terminal arborizations. The results indicate that the projection fields of the axon collaterals of principal cells are spatially separated from the dendritic projection fields. This suggests that the output of these principal cells through the collaterals has a functionally different role from the output through the dendrites. The observation that the three types of principal cells differ in the distribution pattern of their axon collaterals in the GrL suggests that there is a functional separation of the sublayers in the GrL.