Abstract
Background: Tumor margin evaluation is of great importance, as the resection margin after CRLM resection is an important factor in the 5-year survival rate of patients undergoing CRLM surgery. The current use of NIR fluorescence imaging in CRLM surgery is based on a qualitative assessment of the visible fluorescence around the resected tumors, however, to date little is known about the exact behavior of the ICG fluorescence rim. To improve the assessment of the fluorescent rim, a standardized method to quantify fluorescence intensities is mandatory. Therefore, we developed a novel method to quantify fluorescence intensities surrounding CRLM by analyzing the distribution of ICG.
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