Distribution of IcaA and IcaB Genes in Biofilm-Producing Methicillin-Resistance Staphylococcus aureus

Abstract

Background: Biofilm-producing methicillin-resistance Staphylococcus aureus (MRSA) strains may reduce the penetration rate of antibiotics which leads to treatment failure; therefore, the aim of our study was to assess the presence of icaA and icaB biofilm-producing genes in clinically isolated MRSA strains. Materials and Methods: All pus samples received in the microbiology laboratory were processed as per the standard microbiological procedure for the isolation of S. aureus. Detection of MRSA is done by the cefoxitin disc diffusion method. Phenotypic detection of biofilm in all MRSA strains was accessed by tissue culture plate (TCP) method and polymerase chain reaction as genotypic method was used for detection of icaA and icaB genes. Results: A total of 222 S. aureus were isolated from pus specimens. Out of these, 127 isolates were MRSA (57%). TCP method demonstrated that 68% of MRSA were biofilm producers, on the other hand, 52 (41%) strains showed the presence of icaA gene, and 39 (31%) strains showed the presence of icaB genes in 127 MRSA strains. Conclusion: The detection of biofilm in S. aureus should be considered as essential for better management of diseases caused by biofilm-producing S. aureus strains.