Distribution of High-Risk Human Papillomavirus Genotypes in Precancerous Cervical Lesions in Ouagadougou, Burkina Faso

Charlemagne Ouédraogo,Théodora Mahoukèdè Zohoncon,Florencia W Djigma,Clarisse T Ouedraogo,Souleymane Ouattara,Dorcas Obiri-Yeboah,Djénéba Ouermi,Prosper Bado,Esther M A Traoré,Olga Lompo,Jacques Simpore,Simon A Akpona

doi:10.4236/ojog.2016.64025

Charlemagne Ouédraogo, Théodora Mahoukèdè Zohoncon + Show 10 more

Open Access

https://doi.org/10.4236/ojog.2016.64025

Copy DOI

Abstract

Aims: We aimed at identifying the high-risk HPV genotypes associated with high-grade dysplastic cervical lesions in Burkina Faso. The available vaccines to Burkina Faso only protect against two high risk HPV genotypes: HPV 16 and 18. Are the genotypes identified in the high-grade precan-cerous lesions in this survey covered by the available vaccines? Methods: The detection and genotyping of high-risk HPV have been conducted based on 118 formalin-fixed and paraffin-embedded archived tissues using the “HPV Genotypes 14 Real-TM Quant” (Sacace biotechnologies&reg, Italy) kit allowing for the detection of fourteen high-risk HPV genotypes: HPV 16, 31, 18, 39, 45, 59, 33, 35, 56, 68, 51, 52, 58 and 66. Results: The prevalence of high-risk HPV infections was 48.8% based on the appropriate PCR results (21/43). The most common HPV genotypes were HPV 39 (21.7%), HPV 35 (13.0%) and HPV 45 (13.0%). Two cases of multiple infections between HPV 39 - 45 and HPV 39 - 59 have been observed. HPV 16 was not detected in this study. Conclusions: We noted a high prevalence rate for HPV 39, HPV 35 and HPV 45, which are not covered by the commercial vaccines. We also found that the prevalence of HPV 18 was very low in this study and HPV 16 was not detected.

Highlights

Human Papillomavirus (HPV) is involved in cervical cancer, cancers of the anogenital and oropharyngeal areas, and benign lesions such as condylomata acuminata and warts [1] [2]
In addition to our previous research [7]-[11], this study aims to identify the High-risk Human Papillomavirus (HR-HPV) genotypes associated with high-grade dysplastic cervical lesions in Burkina Faso
This study involved 118 tissues diagnosed as high-grade precancerous lesions according to the minutes of the histological result

Summary

Introduction

Human Papillomavirus (HPV) is involved in cervical cancer, cancers of the anogenital and oropharyngeal areas, and benign lesions such as condylomata acuminata and warts [1] [2]. It is the most common sexually transmitted infection (STI) in the world with 660 million people infected according to the World Health Organization [3]. Genotypes of HPV 16 and 18 are most commonly found in cases of cervical cancer in the world and in Europe [6] This generality is not a proven certainty in Africa and in Burkina Faso which has not yet a large-scale mapping of circulating genotypes. In addition to our previous research [7]-[11], this study aims to identify the HR-HPV genotypes associated with high-grade dysplastic cervical lesions in Burkina Faso

Methods

Results

Discussion

Conclusion