Abstract
The polypeptide composition of cytoplasts and karyoplasts prepared from HeLa cells prelabelled with [ 35S]-methionine and enucleated with Cytochalasin B has been analyzed using high resolution two dimensional gel electrophoresis (IEF and NEPHGE). Of the 259 major proteins followed in this study we have identified 73 polypeptides (30 acidic (IEF) and 43 basic (NEPHGE)) that are present mainly in karyoplasts. One of these polypeptides (IEF 49) has previously been shown to be a polypeptide marker for cycling cells, A total of 59 polypeptides (27 acidic and 32 basic) were found to be present mainly in cytoplasts. Many polypeptides (109 acidic and 18 basic) including γ and β-actin (60% in cytoplasts), β-tubulin (60% in cytoplasts), vimentin (75% in cytoplasts) and α-actinin (65% in cytoplasts) were found to be present in both cellular fragments. These results could be of value in assigning the cellular distribution of potential regulatory proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
