Abstract
Sulphur is one of the essential plant nutrients and it contributes to yield and quality of crops. Thiobacillus play an important role in sulphur oxidation in soil and domestic wastewater.The present study is sought to understand the frequency of Thiobacillus in sewage water samples collected from hospital and domestic area.Distribution of sulphur oxidizing bacteria were analysed by using modified Thiobacilli agar. Five isolates were selected and analysed their selected putative virulence properties phenotypically.Also they were showed biocompatible within the group of the Thiobacilli.The extracellular products (culture supernatant) of Thiobacilli showed a substantial level of haemolytic activity and antagonistic activity. Among the isolates from sewage sources, TB-D1 and TB-D2 produced higher level of bacteriocin against pathogenic bacteria due to the formation of maximum inhibition zone. It reveals that the bacteriocin from Thiobacillus showed the elevated antagonistic activity against most of the pathogenic bacteria, as we tested.The strains TB-D4 and TB-D5 showed beta and alpha haemolysis, it might have virulent properties and cause infection in human and animals.The strain TB-D5 showed the maximum inhibitions while compared with others. It is visibly showing presence of virulence factors in the extracellular metabolites of Thiobacilli.
Highlights
Sulphur is considered the fourth major plant nutrient after N, P and K and is one of the sixteen elements which are essential for the growth and development of plants
The role of chemolithotrophic bacteria of the genus Thiobacillus in this process is essential.Organisms belonging to the group of colorlesssulfur bacteria oxidize sulfide to elemental sulfur under oxygen limiting conditions
The major contribution of T. ferrooxidans to metal extraction is its ability to attack sulphide containing minerals and convert the insoluble sulphides of metals such as copper, lead, zinc or nickel to their soluble metal sulphates.An alternative way to replace chemical methods in removing heavy metals is microbial leaching with Acidithiobacillussp. (Tyagi and Couillard, 1989; Tyagi et al, 1993; Blasis et al, 1993; Couillard and Mercier, 1993; Sreekrishnan et al, 1996)
Summary
Sulphur is considered the fourth major plant nutrient after N, P and K and is one of the sixteen elements which are essential for the growth and development of plants. The role of chemolithotrophic bacteria of the genus Thiobacillus in this process is essential.Organisms belonging to the group of colorlesssulfur bacteria oxidize sulfide to elemental sulfur under oxygen limiting conditions. Based on this feature many researchers worked for biological oxidation using various types of microorganisms (Gadre, 1989). (Tyagi and Couillard, 1989; Tyagi et al, 1993; Blasis et al, 1993; Couillard and Mercier, 1993; Sreekrishnan et al, 1996) This method shows several advantages over chemical methods extraction, lower acids and alkali consumption and minimum reduction in sludge nutrients such as N and P (Tyagiet al., 1988; Couillard and Mercier, 1993)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
