Distribution of GABA-like immunoreactivity in the song system of the zebra finch

Abstract

GABA-like immunoreactivity (GABA-LIR) was mapped in the male and female zebra finch song system using a polyclonal antibody to GABA. GABA-LIR was found throughout the song system in neurons and neuropil of the robust nucleus of the archistriatum (RA), the higher vocal center (HVC), Area X, the magnocellular nucleus of the neostriatum (MAN), and the dorsomedial portion of the nucleus intercollicularis (DM of ICo). Puncta present in the lateral division of MAN (1MAN) may be local interneurons since the only known afferents of 1MAN are from the dorsolateral nucleus of the anterior thalamus (DLM), which did not appear to have any cell bodies with GABA-LIR. Distinct and dense puncta with GABA-LIR were present in DLM, and may be projections from Area X/lobus parolfactorius (LPO). Dramatic sex differences in GABA-LIR distribution were found. Females did not appear to have any GABA-LIR above background in either RA of HVC. Females also did not appear to have a distinct Area X, although they did have many small, lightly staining cell bodies in the corresponding LPO. The distribution of GABA-LIR and sex differences in its distribution suggests that GABAergic neurons may play a role in the acquisition and/or production of song in the zebra finch.