Distribution of GABAergic premotor nonspiking local interneurones in the terminal abdominal ganglion of the crayfish.

Abstract

The inhibitory neurotransmitter of premotor nonspiking local interneurones in the crayfish terminal abdominal ganglion was investigated physiologically and immunocytochemically. Depolarization of a nonspiking interneurone evoked a hyperpolarization in a uropod motor neurone. The amplitude of hyperpolarization in the motor neurone was gradually decreased under low-calcium/high-magnesium saline. Local pressure injection of gamma-aminobutyric acid (GABA) into the neuropil caused a similar hyperpolarization of the motor neurone. These physiological studies suggested a GABAergic inhibitory interaction between nonspiking interneurones and the motor neurones. Premotor nonspiking interneurones are classified into two subgroups ofposterolateral (PL) and anterolateral (AL) interneurones, and AL interneurones are further divided into three subtypes. A combination of intracellular staining from nonspiking local interneurones with Lucifer yellow and immunocytochemical staining with an antiserum directed against GABA revealed that all the PL interneurones sampled in this study showed GABA-like immunoreactivity. A population of cell bodies (n = 6-11) with a small diameter (15-30 microm) packed together forming a cluster showed GABA-like immunoreactivity, and the cell bodies of most PL interneurones were found in this cluster. To compare the number and the pattern of main branches of PL interneurones, cells were classified into three identifiable sets of interneurones, called PL-1, PL-2, and PL-3. By contrast, about one-half of AL interneurones, especially the third subtype of AL interneurones, which have cell bodies located ventrolaterally in the ganglion, did not show GABA-like immunoreactivity. Furthermore, the position of cell bodies of GABA-immunoreactive AL interneurones was scattered compared to that of PL interneurones.