Distribution of FMRFamide-like immunoreactivity in the brain of the lizard Podarcis sicula

Abstract

The distribution of FMRFamide-like immunoreactive peptides was investigated in the brain of the lizard, Podarcis sicula, using the indirect immunofluorescence technique. The main populations of FMRFamide-immunoreactive cell bodies were located in the forebrain. In the telencephalon, FMRFamide-containing neurons were found both in the pallium and subpallium, namely in the medial cortex, the anterior olfactory nucleus, the nucleus accumbens, the septal nuclei, the nucleus of the medial forebrain bundle, and the nucleus of the diagonal band of Broca. In the diencephalon, a dense accumulation of FMRFamide-immunoreactive neurons was observed in the area preoptica lateralis, the nucleus suprachiasmaticus, the nucleus periventricularis hypothalami, the area lateralis hypothalami, and the dorsal region of the nucleus geniculatus lateralis. In the midbrain, sparse immunoreactive perikarya were found in the tegmentum of the mesencephalon. FMRFamide-immunoreactive fibers were visualized in all regions containing positive cell bodies. In particular, dense bundles of immunoreactive processes were seen in the area preoptica lateralis, in the hypothalamus, and in the median eminence. The tectum and the basal mesencephalon were also densely innervated. Conversely, the caudal brain stem only exhibited scarce immunoreactive processes. The distribution pattern of FMRFamide-immunoreactive neurons in the brain of Podarcis sicula exhibits a number of similarities with that reported in mammals, but significantly differs from that reported in amphibians and fish, suggesting that the neuromodulatory functions of FMRFamide may have diverged during the emergence of terrestrial life.