Distribution of Flavonoids and Cyclohexenyl Chalcone Derivatives in Conventional Propagated andIn Vitro-Derived Field-GrownBoesenbergia rotunda(L.) Mansf.

Boon Chin Tan,Norzulaani Khalid,Siew Kiat Tan,Sher Ming Wong,Noorsaadah Abd Rahman,Nabeel Ata

doi:10.1155/2015/451870

Boon Chin Tan, Norzulaani Khalid + Show 4 more

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https://doi.org/10.1155/2015/451870

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Abstract

The distribution patterns of flavonoids and cyclohexenyl chalcone derivatives in conventional propagated (CP) and in vitro-derived (CPA) field-grown plants of an important medicinal ginger, Boesenbergia rotunda, are described. A total of eight compounds were extracted from six organs (rootlet, rhizome, shoot base, maroon stem, stalk, and leaf) of the CP and CPA plants. Five major chromatographic peaks, namely, alpinetin, pinocembrin, pinostrobin, 4-hydroxypanduratin A, and panduratin A, were consistently observed by high performance liquid chromatography. Nonaerial organs had higher levels of flavonoids than the aerial ones for all types of samples. Among the compounds detected, pinostrobin and 4-hydroxypanduratin A were the most abundant flavonoid and cyclohexenyl chalcone derivative, respectively. The distribution and abundance of the bioactive compounds suggested that the shoot base could be more potentially useful for medicinal application than other organs of the plant and may be the site of storage or occurrence of biosynthetic enzymatic activities.

Highlights

Bioactive compounds from rhizome extracts have been identified [2, 3] and classified mainly into two major groups, flavanones and chalcones [4]
The distribution of selected bioactive compounds in conventional propagated (CP) and CPA field-grown plants was investigated in order to evaluate selected biochemical contents and to understand their site of biosynthesis with the aim of producing higher accumulation
Rootlets, rhizomes, and shoot bases were considered as nonaerial organs of CP and CPA fieldgrown plants, whereas leaves, stalk, and maroon stem were classified as aerial organs (Figure 1)

Summary

Introduction

Bioactive compounds from rhizome extracts have been identified [2, 3] and classified mainly into two major groups, flavanones (e.g., alpinetin, pinostrobin, and pinocembrin) and chalcones (e.g., boesenbergin, cardamonin, panduratin A, and 4-hydroxypanduratin A) [4]. We have examined and compared the production of various flavonoids and chalcones produced in conventional propagated (CP) and in vitro-derived (CPA) field-grown plants of B. rotunda. To analyze the metabolic profiles in different tissues of B. rotunda, CPA of 2-3 cm in height and CP plants were cleaned and separated into six organs: rootlets, rhizomes, shoot-base rhizomes, maroon stems, leaf stalks, and leaves (Figure 1).

Results

Conclusion