Abstract
Distribution of F-actin and the membrane skeletal proteins fodrin and ankyrin in gastric parietal cells of the rat was examined by immunofluorescence and immunoelectron microscopic techniques of frozen sectiens. Rats were treated with gastrin or famotidine, a histamine H2 receptor antagonist, to stimulate or inhibit secretion, respectively.F-actin staincd by rhodamine-phalloidin was seen along the apical and basolateral cell surface and in the intracellular canaliculi (IC) . The canalicular staining was more pronounced in gastrin-treated than in famotidine-treated rats. By immunofluerescence microscopy, fodrin was observed along the baso1ateral cell membrane and around IC in famotidine-treated rats; ankyrin coexisted with fodrin only in the basolateral cell membrane. Following treatment with gastrin IC was expanded and fodrin labeling in IC was less intense. Immunoelectron microscopy showed that fodrin and ankyrin were distributed along the baso1ateral cell membrane. Fodrin was also seen densely around IC, which distribution became sparser in stimulated cells. Neither fodrin nor ankyrin was found in the tubulovesicles.The present study showed that distribution of both F-actin and fodrin in IC changed according to the cell's secretory state and this chage might be related to the morphological alterations of the gastric parietal cell during secretion. In addition, coexistence of fodrin and ankyrin was revealed along the basolateral cell membrane.
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