Abstract
Hen oviduct chromatin was digested with DNAase II and two fractions were isolated: MgCl 2-insoluble chromatin and MgCl 2-soluble chromatin. The former contained 14 and 50% of the total DNA after a digestion time of 3 and 30 min, respectively. The fraction was characterized in sucrose gradients by a peak sedimenting at 11 S. In the course of DNAase digestion this fraction lost most of its estrogen receptors as assayed by [ 3H]estradiol exchange reaction. The specific radioactivity of chromatin was particularly low in the 11 S region. The MgCl 2-soluble chromatin contained at most 5.1% of the total DNA. In sucrose gradients the fraction displayed peaks at 4 S and 14 S. After a 30 min DNAase digestion the specific radioactivity of chromatin in this fraction exceeded that of the MgCl 2-insoluble fraction 7.7 fold. Material sedimenting at 14 S and at larger S values was enriched in estrogen receptors The results suggest that estrogen receptors are unevenly distributed on hen oviduct chromain.
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