Abstract
Tissue distribution of the enzymes PAPS:desulfoglucosinolate sulfotransferase (sulfotransferase) and UDP-glucose:thiohydroximate glucosyltransferase (glucosyltransferase) was studied in developing seedlings of Brassica juncea Coss.. Hypocotyls had the highest specific activity of enzymes, however, their total activities were the greatest in the cotyledons. The subcellular distribution of both enzymes was investigated using protoplasts generated from cell suspension cultures derived from hypocotyl explants of B. juncea. After purification of the protoplasts and their subsequent rupture, organelle fractions were obtained by differential centrifugation. Activities of both enzymes were most abundant in the soluble fraction.
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