Distribution of endogenous β-galactoside-specific lectin, fibronectin and type I and III collagens during dermal condensation in chick embryos

Abstract

ABSTRACT The distribution of endogenous β-galactoside-specific lectin, fibronectin, type I and III collagens was studied by the indirect immunofluorescence method during the formation of dermal condensation in the feathered region (dorsal skin) of a chick embryo. Endogenous β-galactoside specific lectin was concentrated in the condensed dermal region, coinciding with the formation of condensation of dermal cells. It was also detected in epidermal placodes. Fibronectin was weakly stained in dermis prior to the formation of dermal condensation but not in epidermis. Condensation of dermal cells resulted in the formation of thicker fibrils of fibronectin in the condensed region. Distribution of type I collagen was found to be very different from that of endogenous β-galactoside-specific lectin and fibronectin. Type I collagen in dermis decreased along with the formation of dermal condensation. Epidermis had no type I collagen. Type Ill collagen was not found in dorsal skin. Relationship found between these distribution patterns and dermal condensation in the embryonic chick skin is discussed.