Distribution of cytochrome P450 1A1 and 1B1 genes single nucleotide polymorphism on the breast cancer patients

Abstract

Objective To discuss the distribution of single nucleotide polymorphism (SNPs) of the gene of cytochrome P450 (CYP) P1A1 (rs4646903) and CYP1B1 (rs1056836) on breast cancer patients and its relation between clinical pathology. Methods A case-control study were performed to analyze 337 breast cancer patients and 469 healthy individuals taking physical examination in West China Hospital of Sichuan University from September 2012 to February 2016. The direct sequencing method was used to analyze the distribution of SNPs in the gene of CYP1A1 (rs4646903) and CYP1B1 (rs1056836) , and the disparity of clinical pathological characteristics in patients with different genotype among the breast cancer population were detected. Results There was no statistic difference of the distribution of CYP1A1 (rs4646903) in genotype between the two groups (χ2=0.409, P>0.05) . There was a remarkable difference of the distribution of CYP1B1 (rs1056836) in genotype between the two groups (χ2=0.009, P<0.01) . In 337 cases of breast cancer patients, the distribution in the polymorphism of CYP1A1 gene (rs4646903) was not associated with the clinical pathological characteristics of the tumor. The single nucleotide polymorphism of the gene of CYP1B1 (rs1056836) had significant correlation with the metastasis of lymph node and the TNM stage (P< 0.05) . The homozygous mutant genotype was associated with higher negative rate of lymph node transformation and lower TNM stage. Conclusions The single nucleotide polymorphism of the gene of CYP1B1 (rs1056836) is associated with the risk of developing breast cancer. The homozygous mutant genotype in the breast cancer patients, have lower metastatic rate of axillary lymph node and TNM stage. Key words: Breast neoplasms; CYP1A1; CYP1B1; Polymorphism; Onset risk