Abstract
To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the 292 spacer sequences identified in this study, 204 shared 93–100% nucleotide homology to Campylobacter phage D10, 44 showed 100% homology to Campylobacter phage CP39, and 3 had 100% homology with Campylobacter phage CJIE4-5. The remaining 41 spacer sequences did not match with any phages in the database. Based on the results, it was inferred that the FQ-resistant C. jejuni isolates analyzed in this study were potentially resistant to Campylobacter phages D10, CP39, and CJIE4-5 as well as some unidentified phages. These phages should be excluded from cocktails of phages that may be utilized to treat FQ-resistant Campylobacter.
Highlights
IntroductionCampylobacter jejuni causes bacterial gastroenteritis in humans worldwide and is responsible for an estimated 1.3 million cases of diarrhea each year in the United States [1,2]
Campylobacter jejuni causes bacterial gastroenteritis in humans worldwide and is responsible for an estimated 1.3 million cases of diarrhea each year in the United States [1,2].Campylobacteriosis is typically a self-limiting condition, with symptoms usually resolving within a week, but antimicrobial therapy may be necessary in immune-compromised and elderly patients [3,4].Campylobacter spp. have been reported to be resistant to antibiotics including fluoroquinolones, beta-lactams, macrolides, and aminoglycosides [4,5]
We investigated the clustered regularly interspaced short palindromic repeats (CRISPR) systems of FQ-resistant C. jejuni isolates derived from various sources
Summary
Campylobacter jejuni causes bacterial gastroenteritis in humans worldwide and is responsible for an estimated 1.3 million cases of diarrhea each year in the United States [1,2]. Campylobacter spp. have been reported to be resistant to antibiotics including fluoroquinolones, beta-lactams, macrolides, and aminoglycosides [4,5]. Fluoroquinolones (e.g., ciprofloxacin) and macrolides (e.g., azithromycin) are the primary antibiotics used for treatment in humans, and resistance to these classes of drugs in Campylobacter is a significant public health concern [6,7]. Whereas fluoroquinolone resistance develops macrolide resistance in Campylobacter is a gradual process requiring prolonged exposure to the antibiotic [8]. In Campylobacter, quinolone antibiotics exert their effect via binding to and interfering with the function of the DNA gyrase enzyme
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
