Distribution of corticospinal motor fibres within the cervical spinal cord with special reference to the phrenic nucleus: a WGA-HRP anterograde transport study in the cat

Abstract

The anterograde transport of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP) was used to demonstrate the corticospinal fibres which originate in the motor cortex and project to the cervical spinal cord, specifically to the phrenic nucleus, in the cat. Following injections of WGA-HRP into the pericruciate cortex large numbers of fibres were labelled in the contralateral lateral and ventral funiculi and fewer fibres were labelled in the ipsilateral and ventral funiculi. Labelled corticospinal motor fibres entered the gray matter laterally in laminae V and VI and terminated within these two laminae as well as throughout the entire extent of lamina VII. A few labelled fibres were present in medial lamina VIII and also in lamina IX where they were in close association with the phrenic motoneuron pool. Labelling was present in the gray matter at both sides, with a stronger labelling contralaterally. Labelled axons were also seen crossing from each side of the gray matter to the other side. The results suggest that in the cat the corticospinal motor fibres have a wider distribution in the spinal gray matter than has been previously shown, and that corticospinal motor axons may be in direct contact with phrenic motoneurons.