Abstract
The morphological distribution of coniferyl alcohol and coniferaldehyde groups in the cell wall of spruce wood fibrers was determined using staining techniques combined with UV-VIS microscopic analysis. Phloroglucinol-HCI was used as a specific stain for coniferaldehyde groups. The distribution of coniferaldehyde groups was measured on 1 μm sections at 550 nm. Coniferyl alcohol groups were transformed into p-dimethylamino-anilide of styryl-glyoxylonitrile and measured at 475nm. The analysis showed that there is an enrichment of coniferyl alcohol and coniferaldehyde groups in the P/S I region of the fibre wall, and coniferyl alcohol groups in the S 3 layer. The high concentration of coniferyl alcohol and coniferaldehyde groups at PIS I coincides with a preferential sulphonation of this region at mild sulphite treatment in chemimechanical pulping.
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