DISTRIBUTION OF CALCIUM OXALATE CRYSTAL IDIOBLASTS IN LEAVES OF TARO (COLOCASIA ESCULENTA)

Abstract

Cleared leaves of taro (Colocasia esculenta) were examined microscopically to determine changes in the distribution of both druse and raphide idioblasts during a late developmental process—leaf unfurling and expansion. Druse crystal idioblasts are small spherical cells found throughout the lamina, mostly in subepidermal areas. Two types of raphide idioblasts were observed in taro leaves: the nondefensive raphide idioblasts, which are elongated cells usually found embedded in tissues of the leaf margins; and the defensive raphide idioblasts, also elongated cells, but usually found suspended between mesophyll cells in leaf airspaces. The densities of both druse and raphide cells were highest at the fully furled stage and least in the mature, unfurled stage, after substantial leaf expansion. During leaf unfurling, the raphide cells showed a bilaterally symmetrical distribution during all stages from fully furled to mature, unfurled leaves. The distribution of druse cells was bilaterally symmetrical during the fully furled and unfurled stages, but, during unfurling, when one half of the lamina is unfurled and the other half is still tightly furled, up to 80% of the druse cells were found on the unfurled half of the lamina.