Distribution of Bm1, a SINE type transposable element, in cecropin B genes of the silkworm, Bombyx mori

Abstract

In order to investigate the distribution of Bm1, a SINE type transposable element, in cecropin B genes of the silkworm, Bombyx mori, a genomic library was first screened with cecropin B cDNA as a probe. Eighteen out of 275 positive clones were selected at random and SalI digestion patterns were compared. Ten clones which showed different patterns were further analyzed by Southern blotting using a cecropin B cDNA fragment encoding exon 1, exon 2 or the entire coding region as probes. The same SalI digested genomic clones were hybridized with a Bm1 probe. Comparison of positive patterns hybridized with the Bm1 probe to those hybridized with the cecropin B probes indicated that all cecropin B-fragments except one fragment had Bm1. The exceptional fragment contained exon 2 only. The results indicate that Bm1 is widely distributed in cecropin B genes.