Abstract
Distribution of total phosphorus (T-P), orthophosphate (Ort-P) and alkaline phosphatase-hydrolyzable phosphorus (ALP-P) were examined in the water of 15 lakes. The ratio of Ort-P to T-P was low in most lakes studied. A considerable proportion of soluble organic phosphorus (62% on average) was ALP-P. The sum of Ort-P and ALP-P, which was thought to be utilized by plankton in lake water, readily accounted for 71% of total soluble phosphorus.Gel chromatography analyses (Sephacryl S-200) revealed that two fractions of phosphorus compounds (high and low molecular weight) existed in the water of L. Inbanuma and L. Kawaguchiko. A portion of the high molecular weight phosphorus of each sample was hydrolyzed by alkaline phosphatase, which indicated that it was composed of a phosphomonoester bound to a high molecular substance such as humic acid.
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