Distribution, diversity and genetic structure of alders (Alnus lusitanica and A. glutinosa) in Spain

Abstract

Alders (Alnus spp., Betulaceae) are keystone pioneer species in riparian habitats. In the Mediterranean Basin, where the diploid Alnus glutinosa coexists with the recently discovered tetraploid A. lusitanica, the distribution, diversity and genetic structure of alder species is unknown. The main objectives of this study were to report the distribution of A. glutinosa and A. lusitanica, quantify the local genetic diversity of populations and explore the presence of putative hybrids resulting from the coexistence of A. lusitanica and A. glutinosa individuals in Spain. We assessed 627 individuals from 22 natural alder populations by combining chromosome counts, flow cytometry and microsatellite markers (SSRs) analyses. The ploidy analysis, validated through chromosome counts, revealed the presence of 92 diploid and 535 tetraploid trees from three A. glutinosa and 18 A. lusitanica pure populations, respectively. Notably, no hybrids were detected, not even in Assa, the only population where both species co-occur. The occurrence of mixed ploidy level in alder is reported for the first time in Spain (Assa, Logroño, within the Ebro basin). SSR analysis revealed robust genetic differentiation between the two species and distinctive patterns of variation within A. glutinosa. The high level of differentiation observed was supported by the most probable population clustering determined through pca and structure analyses, along with the identification of a high number of private alleles. For A. lusitanica, the results revealed three distinct genetic groups, geographically distributed. Positive genetic and geographical distance correlations indicated that differentiation could have been driven by isolation by distance. In light of the results, A. lusitanica is the most representative, widespread and genetically variable alder species in Spain, and A. glutinosa is restricted to the north-east of the country. The Ebro riverbank is a contact zone between A. lusitanica and A. glutinosa species, in which no evidence of hybridization was detected yet. Finally, with focus on management, our findings may suggest considering A. lusitanica as a new species, which should be regulated by the forest reproductive material legislation.