Abstract
Extracellular polymeric substance (EPS), a high molecular weight biological polymer that can bind to a large amount of organic matter and moisture, plays a vital role in the process of sludge formation and structural stability, affecting sedimentation and dewatering performance of sludge. The aim of this study was to observe the changes in the key components of EPS extracted from mechanical dewatered sludge before and after enzyme and thermal pressure treatment. The results showed that the soluble protein content increased by 160 and 110% and the polysaccharide content increased by 180 and 200% after adding 0.03 g/g TSS neutral protease and alpha-amylase, respectively. The effect of compound enzyme treatment was better than that of single enzyme treatment. Furthermore, three-dimensional fluorescence spectroscopy indicated the presence of tryptophan and aromatic-like proteins, and Fourier transform infrared spectroscopy showed that the types of functional groups exhibited a sharp decrease in the loosely bound-EPS layer after thermal pressure treatment.
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