Abstract
Objective: The widespread prevalence of Giardiasis is a public health problem worldwide, and it is also common among Yemeni children. Hence, this work aims to identify the prevalent Giardia lamblia infection and risk factors among children admitted to health care centers in Amran governorate in Yemen.
 Methods: Three hundred and thirty-four stool samples were collected from infected children between March and July 2019 and the parasite is identified by light-microscope according to standard parasitology methods. Also, relevant data was obtained using a designed questionnaire.
 Results: The results showed that 181 cases (54.2%) were infected with G. lamblia infection. 57.5% of the infected children were from urban areas, while 42.5% of the cases were from rural areas. The highest incidence of G. lamblia was 60.3% among males compared to 47.5% among females. The results for age, residents and male children aged 8-14 years in both urban and rural areas recorded the highest prevalence of G. lamblia infection while the lowest was among females between 1 and 7 years and 8-14 years old, respectively, in the rural and urban area.
 Conclusion: In conclusion, as described in this work, multisectoral efforts are needed that include hygiene practices, personal hygiene habits, the provision of safe drinking water and the provision of sanitation systems to efficiently reduce this infection from all governorates of Yemen.
 Peer Review History: 
 Received 22 May 2020; Revised 14 June; Accepted 3 July, Available online 15 July 2020
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 Received file 
 
 Average Peer review marks at initial stage: 6.0/10
 Average Peer review marks at publication stage: 7.5/10
 Reviewer(s) detail:
 Name: Prof. Dr. Hassan A.H. Al-Shamahy
 Affiliation: Sana'a University, Yemen
 E-mail: shmahe@yemen.net.ye
 
 Name: Dr. Mahmoud S. Abdallah
 Affiliation: University of Sadat city, Egypt
 E-mail: dr_samy777@yahoo.com
 
 Comments of reviewer(s): 
 
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Highlights
Three hundred and thirty-four [334] stool specimens were chosen of children from Amran governorate, 160(47.9%) specimens were from an urban area and 174(52.8%) specimens from a rural area (Figure 1)
The current results related to gender and age showed that the highest prevalence of G. lamblia infection was reported among the age group of 8 to14 years (81.90%) in males followed by 61.9% was among the age group of 1-7 years in females recorded urban area
This study revealed a high frequency of G. lamblia infection among children compared to other studies in other governorates’ in Yemen where it ranged between 23.6% and 23.94% in Ibb8,13, 3% in Al-Mahweet9, 19.17% in Hadramowat10, and between 16.7% and 17.7% in Sana’a11,14
Summary
Study Design and Area This study is a cross-section study carried out in the medical laboratory at 22 May hospital in Amran City, Yemen, during the period from March to July 2019. This investigation was applied at Amran governorate in both of urban and rural areas; about 50 km north of Sana'a, the capital of Yemen. Data Collection Prior to specimen collection, the objectives of the study were explained briefly to all children. Sample Collection and Examination A total of three hundred and thirty-four [334] stool specimens were collected from infected children in clean plastic containers (60 ml) and labeled. The wet mount preparation was used after formalether sedimentation technique according to Cheesbrough
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