Distribution and location of Daxx in cervical epithelial cells with high risk human papillomavirus positive

Shuang-Yang Tang,Le Li,An-Yuan Liu,Yao-Lin Li,Min-Jun Yu,Yan-Ping Wan

doi:10.1186/1746-1596-9-1

Abstract

AimsTo provide the basis for further exploring the effect and its mechanism of Death domain associated protein (Daxx) on the progress of cervical carcinoma induced by human papillomavirus (HPV), the distribution and location of Daxx in cervical carcinoma with high risk HPV(HR-HPV) positive was analyzed.MethodsThe samples of normal cervical epithelial cells, cervical intraepithelial neoplasia grade I (CINI), CINII CINIII and cervical cancers were collected. Immunohistochemistry assay was used to analyze the distributions and locations of Daxx in the cervical tissue. Indirect immunoinfluorescence test was utilized to observe the locations of Daxx in Caski cells with HPV16 positive.ResultsUnder the light microscopy, the brown signals of Daxx distributed in the nuclei of normal cervical epithelial cells; Daxx mainly distributed in nuclear membrane and there were a small amount of Daxx in the nuclei in CINI. Daxx intensively distributed in the cytoplasm and cell membrane in CINII, CINIII and cervical cancer. Under fluorescent microscopy, the distribution and location of Daxx in Caski cells was similarly to that in cervical cells of CINII, CINIII and cervical cancer.ConclusionIn the progress of the cervical cancer, Daxx gradually translocates from nucleus into nuclear membrane, cytoplasm and cell membrane. Daxx locates in the cytoplasm and cell membrane in CINII, CINIII and cervical cancer.Virtual slidesThe virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/4671548951113870.

Highlights

Death domain associated protein (Daxx) is a highly conserved nuclear protein playing an important roles in the transcriptional control, carcinogenesis, resistance to virus infection and so on [1]
We found that HPV16 E6 could translocate some Daxx from the nucleus to the cytoplasm when HPV16 E6 over-expressed in HeLa cells [9], which might be related to the normal form change of PML-NBs
Expression of Daxx in cervical cells infected by HR-human papillomavirus (HPV) Under the optical microscope, Daxx was tan in cervical tissue using immunohistochemical test (Figure 1)

Summary

Introduction

Death domain associated protein (Daxx) is a highly conserved nuclear protein playing an important roles in the transcriptional control, carcinogenesis, resistance to virus infection and so on [1]. The most obvious depositories of Daxx are promyleocytic leukaemia protein (PML) nuclear bodies (PML-NBs), that. About 10% HPV16 infections would be latent, despite these mechanisms are unclear, in which Daxx within PMLNBs is likely to play a role that is unclear. HPV capsid protein L2 can induce the restructuring of PML-NBs, and increase the gathering of Daxx co-locating with PML [7]. HPV18 E6 can co-locate with PML and promote its degradation by proteasome pathway, and inhibit the cell aging caused by PML [8]. Daxx locating in PML-NBs may play a very important role in intracellular defense against viral infection

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Results

Conclusion