Abstract
Abstract Bioassay/RIA-based screening of seeds of seven species of the genus Gomphrena revealed the presence of phytoecdysteroids in five of them, but not in G. globosa and G. hybrida . The accumulation of phytoecdysteroids was highest in G. affinis (ca. 8.6 mg ecdysone equivalent/g seed, using the DBL-1 antiserum). The four other ecdysteroid-positive species ( G. canescens, G. cunninghamii, G. dispersa and G. haageana ) contained moderate levels of phytoecdysteroids (1–2 mg ecdysone eq./g). 20-Hydroxyecdysone was the predominant ecdysteroid present in each of these seed extracts, accounting for >90% of the total ecdysteroids. Several of the minor ecdysteroids have been tentatively identified. Among different parts (seeds, roots, stems, leaves and flowers) of G. dispersa and G. haageana , seeds contained the highest, and roots the lowest, levels of phytoecdysteroids. RP-/NP-HPLC separation coupled with monitoring by UV, RIA and bioassay revealed that 20-hydroxyecdysone generally constituted ca. 95% of the total ecdysteroid content, but in a few of the extracts the levels and identities of other ecdysteroids varied significantly.
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