Distribution and drug resistance of 1 701 positive isolates from blood culture samples

Abstract

Objective To investigate the distribution and drug resistance in blood culture isolates in Sichuan Provincial People’s Hospital. Methods A total of 18 943 blood culture samples obtained from January 2011 to November 2013 were performed by automated detection system. The pathogens and the drug resistance of positive isolates were analyzed. Results A total of 1 701 positive strains were isolated with the positive rate of 9.0%. Gram positive cocci, Gram negative bacilli and fungi accounted for 52.3%, 45.8% and 1.9%, respectively. The most frequent isolates were coagulase negative Staphylococcus (CNS, 37.7%) , E.coli (23.6%) , Klebsiella spp. (8.8%) , Enterococcus spp. (7.3%) and S. aureu (7.1%) . The prevalence of E. faecalis strains resistant to high level gentamicin and ampicillin were 71.4% and 0, respectively. The E. faecium isolates showed significantly higher resistant rates to most of the antimicrobial agents than E. faecalis ones.No Staphylococcus spp. strains were found resistant to vancomycin. The drug resistance rate of E. faecium to vancomycin was 5.3%. The resistance rates of E. coli and Klebsiella spp. strains to imipenem were 0.3% and 0.7%, respectively.More than 50% of E. coli strains were resistant to fluoroquinolones. The resistance rates of Entrobacter spp. to imipenem and ertapenem were 10.8% and 19.4%, respectively. More than 80% of Acinetobacter baumannii strains were resistant to carbapenems. Conclusions Mainly gram positive bacteria and gram negative bacilli were isolated from blood culture samples of the Sichuan Provincial People's Hospital in past 3 years, and resistance to antibiotics is common in most blood isolates. Key words: Bacteria; Distribution; Drug resistance; Blood