Abstract
Levels of sodium (Na+), potassium (K+), calcium (Ca+2) and magnesium (Mg+2) ions were determined in the root, leaf and petiole of cultivated Sassurea lappa plant collected from Pakistan Forest Institute, Peshawar. The same ions were also analyzed in root (b) of the same plant collected from Koza Gali, Abbottabad as well as in wild plant root (c) of Kashmir hills. Potassium (K+) and sodium (Na+) in the medicinal plant, S. lappa were determined by using flame photometer (Jenway PFP 7) while Ca+2 and Mg+2 contents were measured by atomic absorption spectrophotometer (Hitachi Z-800). Significant differences were observed among all the tested samples. However, the highest level of K+ was 1886.45 µMg-1 found in cultivated plant petiole and its lowest concentration was 385.351 µMg-1 observed in shoot derived callus (callus a). Sodium content (Na+) was found higher in shoot callus (callus a) while magnesium (Mg+2) concentration was observed high (109.349 µMg-1) in root (b) collected from Koza Gali, Abbottabad and their lower concentrations were 102 µMg-1 and 47.432 µMg-1 found in petiole and wild plant root (root c) respectively. The lowest concentration was found in case of Ca+2 in comparison of other elements. The levels of all the selected elements were highly varied in the anatomical parts of the same cultivated plant, shoot and root derived calli and between the varied regions plant roots except Ca+2. The overall levels of the elements were found in the order: K+ > Na+ > Mg+2 > Ca+2. Keywords: Saussurea lappa; In vitro culture; Calli; Major elements http://dx.doi.org/10.19045/bspab.2019.80087
Highlights
Out of eleven essential elements such as nitrogen, potassium, phosphorus, calcium, sulphur, magnesium, iron, boron, zinc, copper and manganese are absorbed from the soil by plants through their roots, six are used in comparatively larger quantities and are receiving major attention [1]
To know whether the concentration of the selected elements are same or show variation in the same plant parts collected from Pakistan Forest Institute (Peshawar), Abbottabad (Koza Gali) and in wild plant of Kashmir hills
The callus growth was expressed as relative growth rate week-1 using equation (A) as given below: RGR = In (Final weight of callus) – In (Initial weight) / 2 week-1A Plant materials The wild growing plant was collected from Kashmir hills whereas cultivated plant was collected in the same season (18-08-2014) from Pakistan Forest Institute Peshawar and root (b) from Koza Gali, Abbottabad
Summary
Certain elements are well thought-out as desirable for successful growth of plants. Out of eleven essential elements such as nitrogen, potassium, phosphorus, calcium, sulphur, magnesium, iron, boron, zinc, copper and manganese are absorbed from the soil by plants through their roots, six are used in comparatively larger quantities and are receiving major attention [1]. The callus growth was expressed as relative growth rate week-1 using equation (A) as given below: RGR = In (Final weight of callus) – In (Initial weight) / 2 week-1A Plant materials The wild growing plant was collected from Kashmir hills (root c) whereas cultivated plant (root a, leaves and petiole) was collected in the same season (18-08-2014) from Pakistan Forest Institute Peshawar and root (b) from Koza Gali, Abbottabad. Inoculation From micropropagated plant, shoot and root explants were excised with the help of sterilized cutter and inoculated by using forceps in 100 ml flasks containing MS medium fortified with 2-4 D + Kn
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