Distribution and co-localization of 5-hydroxytryptamine, thyrotropin-releasing hormone and substance p in the cat medulla

Abstract

This study demonstrates the co-existence of three neurochemicals in ventral medullary neurons of the cat utilizing fluorescence immunohistochemistry. Neurons containing 5-hydroxytryptamine, thyrotropin-releasing hormone and substance P were identified within the rostrocaudal extent of the medulla, specifically within the raphe pallidus and raphe magnus and in the reticular formation of the ventrolateral medulla in the nucleus paragigantocellularis lateralis. Within the raphe pallidus the majority of 5-hydroxytroptamine-containing neurons were co-localized with thyrotropin-releasing hormone and substance P. However, in the raphe magnus the majority of stained neurons contained 5-hydroxytryptamine and thyrotropin-releasing hormone but were devoid of substance P. In the ventrolateral medulla two major populations of neurons were identified rostral to the inferior olivary nuclei, one containing 5-hydroxytryptamine and thyrotropin-releasing hormone, while a more lateral group contained substance P alone. More caudally, at the level of the inferior olives, the majority of 5-hydroxytryptamine-containing cells also displayed immunoreactivity for thyrotropin-releasing hormone and substance P. A consistent finding in both the ventromedial and ventrolateral regions of the medulla was a population of 5-hydroxytryptamine-containing cells which did not stain for either thyrotropin-releasing hormone or substance P. The functional role of co-localized neurochemicals remains unknown but co-existence of neurotransmitter substances in medullary neurons may allow for specific and multiple actions in the spinal cord.