Distribution and classification of the extracellular matrix in the olfactory bulb

Andrea Hunyadi,Markus Morawski,Éva Rácz,David Lendvai,Clara Matesz,Botond Gaál,Ildikó Wéber,Katja Reimann,Alan Alpar,Zoltan Meszar

doi:10.1007/s00429-019-02010-8

Andrea Hunyadi, Markus Morawski + Show 8 more

Open Access

https://doi.org/10.1007/s00429-019-02010-8

Copy DOI

Abstract

Extracellular matrix (ECM) became an important player over the last few decades when studying the plasticity and regeneration of the central nervous system. In spite of the established role of ECM in these processes throughout the central nervous system (CNS), only few papers were published on the ECM of the olfactory system, which shows a lifelong plasticity, synaptic remodeling and postnatal neurogenesis. In the present study, we have described the localization and organization of major ECM molecules, the hyaluronan, the lecticans, tenascin-R and HAPLN1 link protein in the olfactory bulb (OB) of the rat. We detected all of these molecules in the OB showing differences in the molecular composition, staining intensity, and organization of ECM between the layers and in some cases within a single layer. One of the striking features of ECM staining pattern in the OB was that the reactions are shown dominantly in the neuropil, the PNNs were found rarely and they exhibited thin or diffuse appearance Similar organization was shown in human and mice samples. As the PNN limits the neural plasticity, its rare appearance may be related to the high degree of plasticity in the OB.

Highlights

The olfactory system, by monitoring odorant molecules of the environment, influences social and sexual behavior
The most typical form was a diffuse staining in the neuropil which, depending on the type of reaction contained small, ring-like structures representing the axonal coats (Brückner et al 2008) and darkly stained dots corresponding to the nodal Extracellular matrix (ECM) (Bekku et al 2009; Bekku and Oohashi 2010)
Extracellular matrix became an important player over the last few decades when studying the plasticity and regeneration of the central nervous system

Summary

Introduction

The olfactory system, by monitoring odorant molecules of the environment, influences social and sexual behavior. Sensory neurons in the olfactory epithelium express various odorant receptors and the central processes of these cells. The neuronal network of the olfactory bulb is continuously reorganized throughout life using two mechanisms. The structure of the olfactory neuronal network is modified by the incorporation of newborn neurons arriving from the subventricular zone (Luskin 1993). These processes result in a high degree of neural plasticity, the underlying molecular mechanism is not fully understood. The aim of the present study is to describe the molecular composition and organization of these ECM molecules in various layers of the olfactory bulb in the rat. In the case of some molecules, we extended the study to human and mices samples

Objectives

Methods

Results

Conclusion