Abstract

Mertz, Dan. (U. Missouri, Columbia.) Distribution and cellular localization of ascorbic acid oxidase in the maize root tip. Amer. Jour. Bot. 48(5): 405–413. Illus. 1961.—Ascorbic acid oxidase has been identified by its various properties in homogenates of the maize root tip. Following centrifugal fractionation of the gross homogenate, approximately 30% of the total oxidase activity was found in the soluble fraction, while roughly 70% was bound to the cell wall debris. Quantitative measurements of serial segments revealed that the bound oxidase per cell increases with cell elongation. The very striking parallelism of the bound oxidase activity with that of cell elongation suggests that ascorbic acid oxidase may in some way participate in growth of the cell wall, and have very little to do with cellular respiration.

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