Distribution and Appearance of Ki-67, IL-1α, IL-10, and PGP 9.5 in Reinke's Oedema-Affected Larynx Tissue Compared with Control Tissue.

Vita Konopecka,Dins Sumerags,Gunta Sumeraga,Mara Pilmane

doi:10.3390/life11121379

Vita Konopecka, Dins Sumerags + Show 2 more

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https://doi.org/10.3390/life11121379

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Abstract

Smoking, laryngopharyngeal reflux, and vocal fold abuse can promote the development of Reinke’s oedema, leading to vocal fold dysfunction and injury. The aim of the work was to investigate the appearance and distribution of proliferation marker Ki-67 (Ki-67), interleukin 10 (IL-10), interleukin 1 alpha (IL-1α), and protein gene peptide 9.5 (PGP 9.5) in Reinke’s oedema-affected larynx tissue. Methods: A routine histological and immunohistochemical Reinke’s oedema and control group patient analysis was conducted. We used the biotin–streptavidin biochemical method to detect Ki-67, IL-10, IL-1α, and PGP 9.5 The semiquantitative grading method was used to evaluate immunoreactive cells’ appearance and local distribution. A Mann–Whitney U test and Spearman’s rank coefficient were performed. Results: A low positive correlation between IL-1α epithelial and subepithelial immunoreactive cells in the patient group was found. Mann–Whitney U tests revealed significant patient and control group immunoreactive marker differences. All examined markers showed a higher number of immunoreactive structures in the patient group. Conclusions: Intensive proliferation of the surface epithelium was observed in patient tissues. The notable increase in IL-10 positive structures indicates the dominant anti-inflammatory tissue response. An increased number of IL-1α structures in the larynx epithelium and subepithelium in the patient group is linked to inflammation, proliferation, and tissue remodelling. The PGP 9.5 expression increase is involved in the morphopathogenesis of Reinke’s oedema.

Highlights

Published: 10 December 2021Reinke’s oedema (RE) is the benign diffuse swelling of the vocal folds, known as various polypoid degeneration or smokers’ polyps
We evaluated seven larynx tissue samples that were obtained from the control group during postmortem autopsies
Intraepithelial infiltration was detected in three patient samples, while the infiltration in the control group was not predominant (Figure 1A,B)

Summary

Introduction

Reinke’s oedema (RE) is the benign diffuse swelling of the vocal folds, known as various polypoid degeneration or smokers’ polyps. These lesions can be unilateral as well as bilateral [1]. Changes appear in the superficial lamina propria (SLP), called Reinke’s space, which has an important functional role in vocal fold vibrations as well as voice production [3]. An accumulation of the oedematous transudate in Reinke’s space can lead to varying levels of dysfunction of the vocal folds. Reinke’s oedema causes the voice to become hoarse, which causes patients to complain of vocal problems [2,5]

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